Introduction to multiplex immunohistochemistry

For the development of new immunotherapies, it is essential to research the tumor microenvironment. Immunohistochemistry is a versatile technique for studying the expression, distribution and activation of proteins in situ. For the detection of antigens, specific antibodies are used on thin cross-sections made of shock-frozen or formaldehyde-fixed, paraffin-embedded tissue. The visualization of the antigen is Read More

Changes to Recommended Western Blotting Protocols

Cell Signaling Technology (CST) antibodies are validated utilizing our really useful western blotting protocol, developed and optimized by CST scientists. All knowledge proven on product webpages and datasheets was generated utilizing these protocols. We strongly suggest following our protocols to obtain optimum outcomes with all of our antibodies. Below are just a few examples of the results Read More

Lab-on-a-Chip-Based PCR-RFLP Assay for the Detection of Malayan Box Turtle (Cuora amboinensis) in the Food Chain and Traditional Chinese Medicines.

The Malayan field turtle (Cuora amboinensis) (MBT) is a weak and protected turtle species, however it’s a profitable merchandise in the unlawful wildlife commerce as a result of of its nice attraction as an unique meals merchandise and in conventional medication. Although a number of polymerase chain response (PCR) assays to determine MBT by numerous Read More

Application of lab-on-a-chip multiplex cassette PCR for the detection of enterohemorrhagic Escherichia coli.

Fast molecular detection strategies profit from ready-to-run lab-on-a-chip molecular assays with minimal preparation time. Detection effectivity of such strategies can enhance if a number of targets are detected concurrently per given response. Detection of meals pathogens, i.e. Escherichia coli (E. coli), is mostly carried out in two levels with the detection of a number of Read More

Within-Subject Performance on a Real-Life, Complex Task and Traditional Lab Experiments: Measures of Word Learning, Raven Matrices, Tapping, and CPR.

In this knowledge report, we describe a three-session experiment spanning six months. Several well-controlled laboratory duties (Word Learning, Raven Matrices, and Tapping) and Cardiopulmonary Resuscitation (CPR), a complicated however well-defined real-world process, have been administered. Data are reported from 50 members for the primary session, 40 for the second, and 34 for the third. CPR Read More